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Hexokinases phosphorylate glucose to produce glucose-6-phosphate the first step in most glucose metabolism pathways This gene encodes hexokinase 2 the predominant form found in skeletal muscle It localizes to the outer membrane of mitochondria Expression of this gene is insulin-responsive and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells
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An analog of oleic acid in which the COOH group is replaced by trifluoromethyl ketone; it’s a potent inhibitor of FAAH, in both human and rat; in transfected COS-7 cells, 10 µM OTK inhibits 95.7% of human FAAH activity and 94.8% of rat FAAH activity
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Recombinant human hexokinase-1 (HK1) is a His-tagged protein expressed in E. coli and supplied lyophilized. The enzyme catalyzes phosphorylation of hexose sugars to their 6-phosphate derivatives and is provided for enzymatic assays, structural studies, and antibody production applications.
N-terminal His tag for purification and detection.
Expressed in E. coli and lyophilized for stability.
Supplied as 50 μg amount.
Molecular weight ~55.9 kDa.
Purity >95% by reducing SDS-PAGE.
Low endotoxin level <1 EU/μg (LAL).
Formulated in Tris/PBS-based buffer with 6% trehalose.
Store at -20°C; aliquot and freeze at -80°C for long-term storage.
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Glucose for Interference Testing is a concentrated glucose solution (250 mg/mL) for spiking into specimens to study the interference of D-glucose in various assays For in vitro research use only
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